期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 44, 期 3, 页码 413-417出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-017-1904-5
关键词
Metabolic footprinting; Unfolded protein response; Clonal variation; Bioprocessing; Recombinant protein
资金
- Bioprocessing Research Industry Club (BRIC) [BBF0049071]
- BBSRC [BB/F004907/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F004907/1] Funding Source: researchfish
Heterologous protein production in the yeast Pichia pastoris can be limited by biological responses to high expression levels; the unfolded protein response (UPR) is a key determinant of the success of protein production in this organism. Here, we used untargeted NMR metabolic profiling (metabolomics) of a number of different recombinant strains, carried out in a miniaturized format suitable for screening-level experiments. We identified a number of metabolites (from both cell extracts and supernatants) which correlated well with UPR-relevant gene transcripts, and so could be potential biomarkers for future high-throughput screening of large numbers of P. pastoris clones.
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