期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 45, 期 1, 页码 43-51出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-017-1990-4
关键词
5-Aminolevulinic acid; Polyhydroxyalkanoates; Escherichia coli; Coproduction; Single-cell biorefinery
资金
- National Natural Science Foundation of China [31670047, 31370085]
Single-cell biorefineries are an interesting strategy for using different components of feedstock to produce multiple high-value biochemicals. In this study, a strategy was applied to refine glucose and fatty acid to produce 5-aminolevulinic acid (ALA) and polyhydroxyalkanoates (PHAs). To express the ALA and PHAs dual-production system efficiently and stably, multiple copies of the poly-beta-3-hydroxybutyrate (PHB) synthesis operon were integrated into the chromosome of Escherichia coli DH5 alpha Delta poxB. The above strain harboring the ALA C5 synthesis pathway genes hemA and hemL resulted in coproduction of 38.2% PHB (cell dry weight, CDW) and 3.2 g/L extracellular ALA. To explore coproduction of ALA and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), the PHBV synthetic pathway was also integrated into engineered E. coli and coexpressed with hemA and hemL; cells produced 38.9% PHBV (CDW) with 10.3 mol% 3HV fractions and 3.0 g/L ALA. The coproduction of ALA with PHB and PHBV can improve the utilization of carbon sources and maximize the value derived from the feedstock.
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