期刊
JOURNAL OF IMMUNOLOGY
卷 198, 期 10, 页码 3846-3856出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1601852
关键词
-
类别
资金
- Chinese National 973 Program [2015CB554303]
- National Natural Science Foundation of China [81590764, 31370888, 31170848, 31470863]
Natural pathogens, such as viruses, often induce T-dependent and T-independent Ab responses. However, the activation and differentiation of Ag-specific B cells under these conditions had not been examined in detail. In this study, we used bacterial phage Q beta-derived virus-like particles (Q beta-VLPs) as an immunogen to examine the T-independent and T-dependent phases of the response in mice. Using Q beta-specific cell labeling and enrichment methods developed in this study, we were able to characterize the rare Ag-specific B cells in detail. Surprisingly, we found that Q beta-VLPs could induce Bcl-6 expression in pregerminal center B cells independently of T cell help. In addition, Q beta-VLP-induced T-independent responses could lead to isotype-switched and somatically mutated memory B cells. Finally, in contrast to what has been reported with several other Ags, long-lived IgG(+) memory cells were induced by Q beta-VLPs, with IgM(+) memory B cells being produced but only evident for a limited time, suggesting that different types of immunogens may preferentially generate or maintain IgM(+) versus IgG(+) memory B cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据