期刊
DEVELOPMENTAL CELL
卷 33, 期 3, 页码 351-365出版社
CELL PRESS
DOI: 10.1016/j.devcel.2015.03.022
关键词
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资金
- ERC [250176, 260746]
- European Commission GENCODYS
- Max Planck Gesellschaft
- European Research Council (ERC) [250176, 260746] Funding Source: European Research Council (ERC)
Membrane trafficking is key to the cell biological mechanisms underlying development. Rab GTPases control specific membrane compartments, from core secretory and endocytic machinery to lesswell-understood compartments. We tagged all 27 Drosophila Rabs with YFPMYC at their endogenous chromosomal loci, determined their expression and subcellular localization in six tissues comprising 23 cell types, and provide this data in an annotated, searchable image database. We demonstrate the utility of these lines for controlled knockdown and show that similar subcellular localization can predict redundant functions. We exploit this comprehensive resource to ask whether a common Rab compartment architecture underlies epithelial polarity. Strikingly, no single arrangement of Rabs characterizes the five epithelia we examine. Rather, epithelia flexibly polarize Rab distribution, producing membrane trafficking architectures that are tissue-and stage-specific. Thus, the core machinery responsible for epithelial polarization is unlikely to rely on polarized positioning of specific Rab compartments.
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