期刊
INDIAN JOURNAL OF PHARMACOLOGY
卷 51, 期 5, 页码 337-342出版社
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/ijp.IJP_87_17
关键词
CENTA; colorimetric assay; Escherichia coli; extended-spectrum beta-lactamase; nitrocefin; Ocimum sanctum; Syzygium aromaticum
资金
- NDVSU, Jabalpur
- College of Veterinary Science and Animal Husbandry, Jabalpur
OBJECTIVE: The inhibitory potential and percent inhibition of Syzygium aromaticum oil and fresh juice of Ocimum sanctum leaves on beta-lactamase enzyme of cecal samples of healthy broilers were studied on samples phenotypically positive for extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli. MATERIALS AND METHODS: Four hundred cecal samples screened for ESBL-producing E. coli were collected from 38 poultry sale outlets located in Jabalpur. The effect of S. aromaticum oil and O. sanctum leaves was seen by colorimetric assay with CENTA and Nitrocefin as chromogenic substrate. RESULTS: Mean absorbance value was inversely propotional to the inhibitory potential. Syzigium aromaticum exhibited 0.4 +/- 0.02 and 0.41 +/- 0.03 mean absorbance value, 28 per cent and 27 per cent of inhibition with CENTA and Nitrocefin respectively. Ocimum sanctum mean absorbance value and per cent inhibition with CENTA and Nitrocefin was 2.03 +/- 0.02 and 10.0 ; 1.97 +/- 0.06 and 10.0 respectively (p>0.05) showing non- significant difference in CENTA and Nitrocefin activity. Tazobactum (100 mu M) as standard control exhibited a mean absorbance value of 0.12 +/- 0.01 and 0.13 +/- 0.01 and percent inhibition of 99.88 and 98 against CENTA and Nitrocefin, respectively. Combination of Ocimum sanctum and Syzigium aromaticum showed range of 1.69 +/- 0.05 to 1.90 +/- 0.08 1.61 +/- 0.06 to 1.92 +/- 0.08 of absorbance value with per cent inhibition of 14 to 15.9 with CENTA and Nitrocefin respectively. CONCLUSION: The results depicted that the inhibition of beta-lactamase enzyme activity with S. aromaticum oil was higher than that of O. sanctum leaf juice, and combination of both the herbs showed not much difference in activity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据