期刊
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES
卷 37, 期 2, 页码 179-184出版社
SPRINGER
DOI: 10.1007/s11596-017-1712-1
关键词
mesenchymal stem cells; interferon gamma; microvesicles; microRNAs
资金
- National Natural Science Foundation of China [81470330]
- Application Fundamental Research Project of Wuhan Science and Technology Bureau [2015061701011623]
Increasing studies have demonstrated that interferon gamma (IFN-gamma), which serves as a critical inflammatory cytokine, is essential to induce the immunosuppressive effects of mesenchymal stem cells (MSCs). However, the mechanisms underlying the enhanced immunosuppressive effects of IFN-gamma-stimulated MSCs (gamma MSCs) are not fully understood. MSC-derived microvesicles (MSC-MVs) have been viewed as potential pivotal mediators of the immunosuppressive effects of MSCs. Moreover, microRNAs (miRNAs) are important regulators of immunological processes and can be shuttled from cell to cell by MVs. The aim of our study was to analyze the the miRNA expression signature of MVs derived from gamma MSCs (gamma MSC-MVs), which may provide better understanding of the immunosuppressive property of their parent cells. Through miRNA microarray and bioinformatics analysis, we found 62 significantly differentially expressed miRNAs (DEMs) in gamma MSC-MVs compared with MSC-MVs. And the potential target genes and signaling pathways regulated by DEMs were predicted and analyzed. Interestingly, many DEMs and predicted signaling pathways had been demonstrated to be involved in immunoregulation. Furthermore, the network between immunoregulation-related pathways and relevant DEMs was constructed. Collectively, our research on the miRNA repertoires of gamma MSC-MVs not only provides new perspectives into the mechanisms underlying the enhanced immunosuppressive property of gamma MSCs, but also paves the way to clinical application of these potent organelles in the future.
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