期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 65, 期 11, 页码 637-653出版社
SAGE PUBLICATIONS LTD
DOI: 10.1369/0022155417732527
关键词
dog; Rab protein; salivary glands; secretory pathway; SNARE complex; syntaxin-6; VAMP4; zymogen granules
类别
资金
- Japan Society for the Promotion of Science (JSPS) [16K08078, 24390050, 25440052]
- joint research program of the Institute for Molecular and Cellular Regulation, Gunma University [15012, 16010]
- Grants-in-Aid for Scientific Research [25440052, 17K08528, 24390050, 16K08078] Funding Source: KAKEN
The comparative structure and expression of salivary components and vesicular transport proteins in the canine major salivary glands were investigated. Histochemical analysis revealed that the morphology of the five major salivary glandsparotid, submandibular, polystomatic sublingual, monostomatic sublingual, and zygomatic glandswas greatly diverse. Immunoblot analysis revealed that expression levels of alpha-amylase and antimicrobial proteins, such as lysozyme, lactoperoxidase, and lactoferrin, differed among the different glands. Similarly, Rab proteins (Rab3d, Rab11a, Rab11b, Rab27a, and Rab27b) and soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) proteins VAMP4, VAMP8, syntaxin-2, syntaxin-3, syntaxin-4, and syntaxin-6 were expressed at various levels in individual glands. mmunohistochemistry of Rab3d, Rab11b, Rab27b, VAMP4, VAMP8, syntaxin-4, and syntaxin-6 revealed their predominant expression in serous acinar cells, demilunes, and ductal cells. The VAMP4/syntaxin-6 SNARE complex, which is thought to be involved in the maturation of secretory granules in the Golgi field, was found more predominantly in the monostomatic sublingual gland than in the parotid gland. These results suggest that protein expression profiles in canine salivary glands differ among individual glands and reflect the properties of their specialized functions.
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