期刊
DEVELOPMENTAL CELL
卷 34, 期 3, 页码 373-378出版社
CELL PRESS
DOI: 10.1016/j.devcel.2015.06.003
关键词
-
资金
- NIH [R01GM098766, S10RR26758]
- National Science Foundation Graduate Research Fellowship Program
CRISPR-based technologies have emerged as powerful tools to alter genomes and mark chromosomal loci, but an inexpensive method for generating large numbers of RNA guides for whole genome screening and labeling is lacking. Using a method that permits library construction from any source of DNA, we generated guide libraries that label repetitive loci or a single chromosomal locus in Xenopus egg extracts and show that a complex library can target the E. coli genome at high frequency.
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