期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 7, 期 44, 页码 6934-6944出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9tb01956a
关键词
-
资金
- Fundamental Research Funds for the Central Universities
- National Natural Science Foundation of China [21372183, 81571803, 31870997]
- Program for Innovative Teams of Outstanding Young and Middle-aged Researchers in the Higher Education Institutions of Hubei Province [T201702]
- Thousand Talents Plan
- Shanghai Municipal Education Commission Innovative Program [2017-01-07-00-07-E00038]
- Shanghai Science and International Cooperation Program [16410724300]
Precise induction and monitoring of cell apoptosis are significant for cancer treatment. This study aims to develop a gold nanoparticle (GNP)-based nanoprobe, which could simultaneously induce and monitor the apoptosis of melanoma cells in situ and realize a precise control of photothermal therapeutic dose. The GNS-TAT-Cy5 nanoprobe was obtained through the formation of Au-S bonds between GNS-TAT and Cy5-tagged caspase-3 specific peptides (Cy5-DEVD). The fluorescence of Cy5 was quenched by the GNS due to the nanosurface energy transfer effect. Upon laser irradiation, activated caspase-3 cleaved the substrate peptide and Cy5 was released from the nanoprobe, leading to a significant fluorescence turn on signal for sensitive and continuous analysis of caspase 3 activity in vitro and in vivo. Therefore, the GNS-TAT-Cy5 nanoprobe can serve as a precise theranostic platform via regulating the photothermal dose and achieved regulation and detection of apoptosis related to caspase-3 for melanoma.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据