期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 50, 期 2, 页码 69-77出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2015.01.007
关键词
C-type lectin; Cynoglossus semilaevis; Immune effect; Antibacterial; Antiviral
资金
- National Natural Science Foundation of China [31330081]
- National Basic Research Program of China [2012CB114406]
- Taishan Scholar Program of Shandong Province
Many C-type lectins (CTLs) have been identified in teleost, however, the in vivo function of fish Cas is essentially unknown. In this study, we examined the function of a CTL (CsCTL1) from tongue sole. CsCTL1 possesses the conserved EPN motif required for mannose binding in mammals but unknown in function in fish. Recombinant CsCTL1 (rCsCTL1), but not the mutant rCsCTL1M bearing substitutions at EPN, interacted with and agglutinated a limited range of bacteria. The agglutinating ability of rCsCTL1 was abolished in the absence of calcium or presence of mannose. Binding of rCsCTL1 to bacteria promoted phagocytosis and antimicrobial activity of head kidney monocytes. Fish administered with rCsCTL1 exhibited enhanced resistance against bacterial and viral infections. These results provide the first evidence that the EPN site is essential to a fish CTL and that, in addition to antibacterial properties, a fish CTL promotes the immune defense against viral infection as well. (C) 2015 Elsevier Ltd. All rights reserved.
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