期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 48, 期 1, 页码 116-123出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2014.09.011
关键词
Siglec-10; B cells; Monoclonal antibody; Swine
资金
- Spanish Ministry of Science and Innovation (MICINN) [AGL2009-09729, AGL2012-31556]
- Spanish Ministry of Economy and Competitiveness
- MICINN
Siglecs are sialic acid binding Ig-like proteins involved in the control of leukocyte responses. In this study we describe the characterization of a porcine orthologue of Siglec-10. A cDNA clone was obtained from a porcine library which encodes a protein with sequence homology to human Siglec-10. This cDNA codes for a type I transmembrane protein containing four Ig-like domains, a transmembrane region, and a cytoplasmic tail with three tyrosine-based motifs, including a membrane-proximal Grb2-binding motif, and two ITIM motifs. When expressed on transfected cells, porcine Siglec-10 was able to bind red blood cells in a sialic acid-dependent manner. Monoclonal antibodies were developed against this protein and used to examine its cell and tissue distribution in the pig. Siglec-10 was found to be expressed on blood B cells and B cell areas of the spleen and lymph nodes. A weak expression was also detected on monocytes. (C) 2014 Elsevier Ltd. All rights reserved.
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