期刊
DEVELOPMENT
卷 142, 期 9, 页码 1684-1694出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.118216
关键词
Epithelial polarity; E-cadherin; Membrane traffic; AP-1; Caenorhabditis elegans
资金
- Ligue contre le Cancer [22/29/35/72]
- Institut national de la sante et de la recherche medicale (INSERM) Avenir grant [R06488NS]
- French Centre national de la recherche scientifique (CNRS)
- Universite de Rennes 1
- Centre national de la recherche scientifique (CNRS) ATIP/Ligue contre le Cancer grant
E-cadherin (E-cad) is the main component of epithelial junctions in multicellular organisms, where it is essential for cell-cell adhesion. The localisation of E-cad is often strongly polarised in the apico-basal axis. However, the mechanisms required for its polarised distribution are still largely unknown. We performed a systematic RNAi screen in vivo to identify genes required for the strict E-cad apical localisation in C. elegans epithelial epidermal cells. We found that the loss of clathrin, its adaptor AP-1 and the AP-1 interactor SOAP-1 induced a basolateral localisation of E-cad without affecting the apico-basal diffusion barrier. We further found that SOAP-1 controls AP-1 localisation, and that AP-1 is required for clathrin recruitment. Finally, we also show that AP-1 controls E-cad apical delivery and actin organisation during embryonic elongation, the final morphogenetic step of embryogenesis. We therefore propose that a molecular pathway, containing SOAP-1, AP-1 and clathrin, controls the apical delivery of E-cad and morphogenesis.
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