期刊
JOURNAL OF GENERAL VIROLOGY
卷 98, 期 2, 页码 305-310出版社
MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.000685
关键词
transmissible spongiform encephalopathies; prion disease; neurodegeneration; scrapie; microRNA; biomarker
资金
- University of Zaragoza, Spain [UZ2014-BIO-05]
- Ministry of Economy and Competitiveness of the Government of Spain (Fondo Europeo de Desarrollo Regional) [AGL2015-67945-P]
- Fondo de Investigacion en Salud, Instituto de Salud Carlos III
- Fondo Europeo de Desarrollo Regional [PI14/00947]
- Grupo Consolidado (Gobierno de Aragon/Fondo Europeo de Desarrollo Regional) [A17]
Scrapie is a transmissible spongiform encephalopathy (TSE), or prion disease, of sheep and goats. As no simple diagnostic tests are yet available to detect TSEs in vivo, easily accessible biomarkers could facilitate the eradication of scrapie agents from the food chain. To this end, we analysed by quantitative reverse transcription PCR a selected set of candidate microRNAs (miRNAs) from circulating blood plasma of naturally infected, classical scrapie sheep that demonstrated clear scrapie symptoms and pathology. Significant scrapie-associated increase was repeatedly found for miR-342-3p and miR-215p. This is the first demonstration, to our knowledge, of circulating miRNA alterations in any animal suffering from TSE. Genome-wide expression studies are warranted to investigate the true depth of miRNA alterations in naturally occurring TSEs, especially in presymptomatic animals, as the presented study demonstrates the potential feasibility of miRNAs as circulating TSE biomarkers.
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