期刊
JOURNAL OF GENERAL VIROLOGY
卷 98, 期 1, 页码 6-17出版社
MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.000674
关键词
H5N1 influenza virus; polymerase mutation; pigeon
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [15K08497, 15K09576, 15K09586, 15H05287, 15H05295]
- Science and Technology Development Fund in Egypt [3282]
- Grants-in-Aid for Scientific Research [16J00589, 15H05287, 15K08497, 15K09576, 15K09586, 15H05295] Funding Source: KAKEN
Highly pathogenic avian influenza virus H5N1 infects a wide range of host species, with a few cases of sporadic pigeon infections reported in the Middle East and Asia. However, the role of pigeons in the ecology and evolution of H5N1 viruses remains unclear. We previously reported two H5N1 virus strains, isolated from naturally infected pigeons in Egypt, that have several unique mutations in their viral polymerase genes. Here, we investigated the effect of these mutations on H5N1 polymerase activity and viral growth and identified three mutations that affected viral polymerase activity. The results showed that the PB1-V3D mutation significantly decreased polymerase activity and viral growth in both mammalian and avian cells. In contrast, the PB2-K627E and PA-K158R mutations had moderate effects: PB2-K627E decreased and PAK158R increased polymerase activity. Structural homology modelling indicated that the PB1-V3D residue was located in the PB1 core region that interacts with PA, predicting that the PB1 mutation would produce a stronger interaction between PB1 and PA that results in decreased replication of pigeon-derived H5N1 viruses. Our results identified several unique mutations responsible for changes in polymerase activity in H5N1 virus strains isolated from infected pigeons, emphasizing the importance of avian influenza surveillance in pigeons and in studying the possible role of pigeon-derived H5N1 viruses in avian influenza virus evolution.
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