期刊
JOURNAL OF GENERAL VIROLOGY
卷 98, 期 6, 页码 1209-1223出版社
MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.000787
关键词
in ovo; avian influenza virus; CpG DNA; nitric oxide; TLR21
资金
- Margaret Gunn Endowment for Animal Research (University of Calgary) and Agriculture and Agri-Food Canada
- Egyptian government
Cytosine-guanosinedeoxynucleotide (CpG) DNA can be used for the stimulation of the toll-like receptor (TLR)21 signalling pathway in avian species which ultimately leads to up-regulation of gene transcription for pro-inflammatory molecules including nitric oxide and recruitment of innate immune cells. The objective of this study was to determine the antiviral effect of NO, produced in response to in ovo delivery of CpG DNA, against avian influenza virus (AIV) infection. We found that when CpG DNA is delivered at embryo day (ED)18 in ovo and subsequently challenged with H4N6 AIV at ED19 pre-hatch and day 1 post-hatching, CpG DNA reduces H4N6 AIV replication associated with enhanced NO production and macrophage recruitment in lungs. In vitro, we showed that NO originating from macrophages is capable of eliciting an antiviral response against H4N6 AIV infection. This study provides insights into the mechanisms of CpG DNA-mediated antiviral response, particularly against AIV infection in avian species.
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