期刊
MATERIALS TODAY BIO
卷 1, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.mtbio.2019.01.001
关键词
Hydrothermal; MCF-7; HaCaT; Reduced ROS level
资金
- DST, India [M-21-133]
- U.G.C.
- Council of Scientific and Industrial Research (CSIR), India
- Uppsala University
- Swedish Research Council (Vetenskapsradet)
- Stiftelsen f_or Strategisk Forskning (SSF (SUSBATT)
Blue fluorescent hexagonal boron nitride quantum dots (h-BNQDs) of similar to 10 nm size as an effective enhancer for DNA cleavage activity of anticancer drug doxorubicin (DOX) were synthesized using simple one-step hydrothermal disintegration of exfoliated hexagonal boron nitride at very low temperature similar to 120 degrees C. Boron nitride quantum dots ( BNQDs) at a concentration of 25 mu g/ml enhanced DNA cleavage activity of DOX up to 70% as checked by converting supercoiled fragment into nicked circular PBR322 DNA. The interaction of BNQDs with DOX is proportional to the concentration of BNQDs, with binding constant K-b similar to 0.07338 mu g/ml. In addition, ab initio theoretical results indicate that DOX is absorbed on BNQDs at the N-terminated edge with binding energy -1.075 eV and prevented the normal replication mechanisms in DNA. BNQDs have been shown to kill the breast cancer cell MCF-7 extensively as compared with the normal human keratinocyte cell HaCaT. The cytotoxicity of BNQDs may be correlated with reduced reactive oxygen species level and increased apoptosis in MCF-7 cells, which may be liable to enhance the anticancerous activity of DOX. The results provide a base to develop BNQD-DOX as a more effective anticancer drug.
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