期刊
JOURNAL OF FOOD ENGINEERING
卷 193, 期 -, 页码 1-9出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jfoodeng.2016.08.012
关键词
Extrusion; Whey protein; Citrus pectin; Maillard reaction; Conjugate formation; Glycation
资金
- German Ministry of Economics and Technology via AiF (Arbeitsgemeinschaft industrieller Forschungsvereinigungen Otto von Guericke e.V.)
- FEI (Forschungskreis der Ernahrungsindustrie e.V., Bonn) [AIF 18070 N]
To investigate the formation of whey protein-citrus pectin conjugates during extrusion processing, extrusion trials were performed by a co-rotating twin screw extruder. The processing conditions such as mechanical stress, reaction time and temperature, are varied by varying screw configuration (with/without reverse elements), and barrel temperature (80 degrees C-180 degrees C). Biopolymer reactions, such as protein and pectin degradation, protein aggregation as well as conjugation were examined by SDS-PAGE and fluorescence spectroscopy. The highest formation of conjugates was observed in the samples treated with reverse elements and the barrel temperature of 140 degrees C. As a measure for the processing conditions, material temperature at the die T-m, specific mechanical energy SME, and residence time distribution RTD were measured. The analysis of these parameters showed that T-m and SME are of significant influence on conjugate formation and degradation. Nevertheless, further analyses at defined conditions (time, temperature, shear stress) are necessary to get a better insight on the reactions taking place in extrusion. (C) 2016 Elsevier Ltd. All rights reserved.
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