期刊
JOURNAL OF FISH DISEASES
卷 40, 期 10, 页码 1351-1362出版社
WILEY
DOI: 10.1111/jfd.12609
关键词
antibiotic susceptibility; grouper; identification; pathogenicity; Vibrio harveyi
资金
- Chinese Academy of Fishery Sciences [2016HY-ZD0505]
- National Key Technology RD Program [2012BAD17B01]
- Construction Programme for 'Taishan Scholarship' of Shandong Province of China
- Programme for Chinese Outstanding Talents in Agricultural Scientific Research
- Qingdao National Laboratory for Marine Science and Technology [2015ASKJ02]
The hybrid grouper, Epinephelus fuscoguttatus (female) x Epinephelus lanceolatus (male), is a newly bred cultivated marine fish species of high economic value. However, a skin ulcer disease with high mortality has occurred, and the responsible pathogen remains unknown. In this study, we summarized the epidemic status and external signs of this disease. We screened potential pathogens and finally isolated one bacterial strain ML01 from affected fish. We subjected healthy juvenile hybrid groupers to bacterial challenge tests with the isolate by immersion, immersion after dermal abrasion and intraperitoneal injection, respectively. Within 14 days post-infection, the isolate ML01 caused mass mortality of juveniles infected via immersion after dermal abrasion or intraperitoneal injection. Diseased juveniles displayed obvioussigns of skin ulcers. The median lethal dose of ML01 by intraperitoneal injection was 1.10 x 10(5) colony-forming units. ML01 was identified as Vibrio harveyi by bacterial morphology, analytical profile index identification, 16S rDNA sequencing and multilocus sequence analysis. Antibiotic susceptibility tests showed that ML01 was sensitive to ceftriaxone, doxycycline and minocycline. The results of this study suggest that V.harveyi is the causal agent of skin ulcer disease in juvenile hybrid groupers, thus providing a basis for effective control and prevention of this disease.
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