期刊
JOURNAL OF FISH DISEASES
卷 40, 期 8, 页码 1089-1101出版社
WILEY
DOI: 10.1111/jfd.12584
关键词
age; barramundi; betanodavirus; NNV; susceptibility
资金
- University of Bergen, Cargill Aqua Nutrition, Norway
- research group Reproduction and development biology, Havforskningsinstituttet
Age-dependent susceptibility to nervous necrosis virus (NNV) was demonstrated for barramundi (Lates calcarifer). The experiment used juvenile barramundi produced from a single spawning that were challenged consecutively by immersion with a redspotted grouper nervous necrosis virus (RGNNV) isolate. The dose and environmental conditions (35ppt salinity and 30 degrees C) were constant. Fish and water were sampled longitudinally for histopathology and RT-qPCR analysis to examine the evolution of the disease, virus replication, immune response and release of virus into water. Viral nervous necrosis (VNN) disease occurred in barramundi challenged at 3 and 4weeks of age while fish challenged at 5, 7 and 9weeks of age developed subclinical infection. Replication of NNV occurred faster and the concentration of virus reached higher concentrations in the younger fish with clinical disease. Virus isolation and qPCR tests indicated that infectious NNV was released from carcasses into water when fish were affected with clinical disease but not when NNV infection was subclinical. Based on these observations, we consider that carcasses from clinically infected fish have a potentially important role in the horizontal transmission of NNV, and barramundi juveniles should be protected from exposure to NNV until they are 5weeks of age and reach the disease resistance threshold.
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