Three MYB genes co-regulate the phloem-based defence against English grain aphid in wheat

Plant phloem-based defence (PBD) against phloem-feeding insects is characteristic of the sieve occlusion by phloem lectins and beta-1,3-glucan callose, both of which are produced under regulation by ethylene and MYB transcription factors. Wheat PBD requires beta-1,3-glucan synthase-like proteins GSL2, GSL10, and GSL12, and may also require insect-resistant mannose-binding lectins Hfr-1 and Wci-1, which can accumulate in the phloem upon aphid feeding. This study elucidates whether any of the 73 MYB genes identified previously in the common wheat Triticum aestivum genome plays a role in wheat PBD activation with regard to the GSLs and lectins. Wheat MYB genes TaMYB19, TaMYB29, and TaMYB44 are highly activated in response to infestation of English grain aphid, and their silencing facilitates aphid feeding on wheat phloem and represses wheat PBD responses. Repressed PBD is shown to decrease aphid-induced callose deposition in wheat leaf epidermis and decrease aphid-induced expression of genes GSL2, GSL10, GSL12, Hfr-1, and Wci-1 in wheat leaf tissues. Based on single gene silencing effects, TaMYB19, TaMYB29, and TaMYB44 contribute 55-82% of PBD responses. However, the contributions of TaMYB genes to PBD are eliminated by ethylene signalling inhibitors, while simultaneous silencing of the three TaMYB genes cancels the tested PBD responses. Therefore, TaMYB19, TaMYB29, and TaMYB44 are co-regulators of wheat PBD and execute this function through crosstalk with the ethylene signalling pathway.