期刊
JOURNAL OF EXPERIMENTAL BIOLOGY
卷 220, 期 13, 页码 2452-2475出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jeb.156646
关键词
Sensory processing; Locomotion; Feeding; Learning and memory; Navigation
类别
资金
- Fundacao para a Ciencia e a Tecnologia (FCT) [SFRH/BPD/75993/2011]
- exploratory grant [EXPL/BEX-BID/0497/2013]
- Cluster of Excellence Cells in Motion (CiM)
- CiM International Max Planck research school (CiM-IMPRS)
- Spanish Ministry of Economy and Competitiveness, 'Centro de Excelencia Severo Ochoa' [SEV-2012-0208]
- CERCA Programme/Generalitat de Catalunya
- 'la Caixa' International PhD Programme
- Spanish Ministry of Science and Innovation [BFU2011-26208]
- Wissenschaftsgemeinschaft Gottfried Wilhelm Leibniz
- State of Sachsen-Anhalt
- Center for Behavioral Brain Sciences Magdeburg
- Otto von Guericke Universitat Magdeburg
- Deutsche Forschungsgemeinschaft [GE1091/4-1]
- European Commission (FP7-ICT project Miniature Insect Model for Active Learning MINIMAL)
- European Research Council (ERC) [2012-StG309832-PhotoNaviNet]
- Swiss National Science Foundation [31003A_169993]
- Deutsche Forschungsgemeinschaft, generation optogenetics [SO1337/2-1, YA272/2-1, PA787/7-1, TH1584/1-1, TH1584/3-1]
- Landesforschungsforderung Hamburg [LFFFV27]
- State of Sachsen-Anhalt, the Center for Behavioral Brain Sciences Magdeburg
- Cluster of Excellence ImmunoSensation
- BadenWurttemberg Stiftung
- Zukunftskolleg of the University of Konstanz
- Swiss National Science Foundation (SNF) [31003A_169993] Funding Source: Swiss National Science Foundation (SNF)
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/75993/2011, EXPL/BEX-BID/0497/2013] Funding Source: FCT
Mapping brain function to brain structure is a fundamental task for neuroscience. For such an endeavour, the Drosophila larva is simple enough to be tractable, yet complex enough to be interesting. It features about 10,000 neurons and is capable of various taxes, kineses and Pavlovian conditioning. All its neurons are currently being mapped into a light-microscopical atlas, and Gal4 strains are being generated to experimentally access neurons one at a time. In addition, an electron microscopic reconstruction of its nervous system seems within reach. Notably, this electron microscope-based connectome is being drafted for a stage 1 larva - because stage 1 larvae are much smaller than stage 3 larvae. However, most behaviour analyses have been performed for stage 3 larvae because their larger size makes them easier to handle and observe. It is therefore warranted to either redo the electron microscopic reconstruction for a stage 3 larva or to survey the behavioural faculties of stage 1 larvae. We provide the latter. In a community-based approach we called the Ol(1)mpiad, we probed stage 1 Drosophila larvae for free locomotion, feeding, responsiveness to substrate vibration, gentle and nociceptive touch, burrowing, olfactory preference and thermotaxis, light avoidance, gustatory choice of various tastants plus odour-taste associative learning, as well as light/dark-electric shock associative learning. Quantitatively, stage 1 larvae show lower scores inmost tasks, arguably because of their smaller size and lower speed. Qualitatively, however, stage 1 larvae perform strikingly similar to stage 3 larvae in almost all cases. These results bolster confidence in mapping brain structure and behaviour across developmental stages.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据