期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 195, 期 -, 页码 266-274出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2016.11.030
关键词
Antifungal; Anti-inflammatory; Vismia guianensis; Prenylated benzophenone, Sporothrix schenckii; Sporotrichosis
资金
- Bioscience and Biotechnology Applied to Pharmacy Pos-graduate Program from Pharmaceutical Sciences Faculty, UNESP, Araraquara, SP, Brazil
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
Ethnopharmacological relevance: Vismia guianensis (Aubl.) Pers. is traditionally used in North and Northeast of Brazil for the treatment of dermatomycoses. Since the strategy associating immunomodulators with antifungal drugs seems to be promissory to improve the treatment efficacy in fungal infections, we aimed to investigate the antifungal activity of V. guianensis ethanolic extract of leaves (VGL) and bark (VGB) against Sporothrix schenckii ATCC 16345 and their antinflammatory activities. Material and methods: The extracts were analyzed by HPLC-DAD-IT MS/MS for in situ identification of major compounds. Antifungal activity was evaluated in vitro (microdilution test) and in vivo using a murine model of S. schenckii infection. The production of TNF-alpha, IFN-gamma, IL-4, IL-10 and IL-12 by measured by ELISA, as well as measured the production and inhibition of the NO after treatment with the plant extracts or itraconazole (ITR). Results: Two O-glucosyl-flavonoids and 16 prenylated benzophenone derivatives already described for Vismia were detected. Both VGL and VGB showed significant antifungal activity either in in vitro assay of microdilution (MIC=3.9 mu g/mL) and in vivo model of infection with reduction of S. schenckii load in spleen. It was also observed a predominance of reduction in the production of NO and the proinflammatory cytokines evaluated except TNF alpha, but with stimulation of IL-10, as evidence of a potential anti-inflammatory effect associated. Conclusion: The results showed that both VGL and VGB have a significant antifungal against S. schenckii and an anti-inflammatory activity. These results can support the use of these extracts for alternative treatment of sporotrichosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据