期刊
JOURNAL OF EQUINE VETERINARY SCIENCE
卷 50, 期 -, 页码 44-51出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jevs.2016.11.008
关键词
Fibroblast-like synoviocytes; Housekeeping gene; Doxycycline; Horse; Stability
资金
- Applied Research Centre, Vice Chancellor for Research of Ferdowsi University of Mashhad [39752]
Real-time quantitative polymerase chain reaction is a powerful and accurate technique to evaluate gene transcription patterns in different cells under biological and pathological conditions. One of the critical steps in the quantitation of transcription profiles is accurate normalization. In this critical step, use of appropriate stable reference gene(s) would be important. The purpose of this study was to identify the most stable housekeeping genes in equine fibroblast-like synoviocytes (FLS) treated with doxycycline from a panel of candidate genes. The gene transcription levels of six commonly used reference genes (GAPDH, ACTB, PPIA, HPRT1, IPO8, and MRPL19) were determined in normal equine FLS or treated by doxycycline and the combination of both. After applying the BestKeeper, geNorm, and NormFinder programs, to this set of genes, there were differences in the ranked order in the three defined groups. But, overall resultants of all three analysis programs determined that the candidate reference genes could be ranked as HPRTI, ACTB, GAPDH, PPIA, MRPL19, and 1P08. To more accurate normalization of gene quantitative data, using of HPRTI, ACTB, and GAPDH combination as reference gene panels is recommended for equine FLS during normal condition or treated by doxycycline. (C) 2016 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据