Characterization of Pb2+ biosorption by psychrotrophic strain Pseudomonas sp I3 isolated from permafrost soil of Mohe wetland in Northeast China

Due to the long and severe winter in Northeast China, wastewater containing lead (Pb) is treated inefficiently, resulting in irregular disposal. In order to solve this problem, a Pb-resistant psychrotrophic bacterium, Pseudomonas sp. I3, was isolated from permafrost soil of Mohe wetland and served as biosorbent for Pb2+ removal under 15 degrees C. The minimum inhibitory concentration of strain I3 for Pb2+ was 7.5 mM, which was higher than that of Escherichia coli DH5 alpha (1.5 mM). However, acid digestion results showed that these two bacteria had a comparable biosorption capacity for Pb2+, suggesting no direct relationship between biosorption ability of bacteria and their metal-resistance. Acid digestion results also proved that intracellular Pb accumulation was mainly contributed to the distinct performance between living and non-living biosorbents, which was further confirmed by the analyses of TEM-EDS: Results of FTIR revealed that functional groups including -CH2, C=O, C-N, N-H, -COO and -SO3 were participated in the biosorption process of the tested biosorbents no matter bacteria were living or not. The effects of environmental factors including pH, temperature, biomass dose, operation time and initial Pb2+ concentration were investigated through a batch of biosorption experiments. The equilibrium data for living and non-living biosorbent were well fitted to Langmuir model with their maximum Pb2+ biosorption capacities of 49.48 and 42.37 mg/g, respectively. The kinetic data for each biosorbent were well described by pseudo-second order kinetic model. Overall, Pseudomonas sp. I3 seemed to be an effective biosorbent for cleansing Pb2+ from contaminated wastewater at low temperature. (C) 2017 Elsevier Ltd. All rights reserved.