期刊
JOURNAL OF DAIRY SCIENCE
卷 100, 期 5, 页码 4014-4024出版社
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2016-11549
关键词
dairy cow; mammary gland; milk lipid; cell death-inducing' DNA fragmentation factor-alpha-like effector C
资金
- National Natural Science Foundation of China [31401109, 31671285]
- University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province, China [UNPYSCT-2015010]
- Young Talents Project of Northeast Agricultural University, China [14QC03]
Adequate lipid synthesis by the mammary gland during lactation is essential for the survival of mammalian offspring. Cell death-inducing DNA fragmentation factor-alpha-like effector C (CIDEC) is a lipid droplet-associated protein and functions to promote lipid accumulation and inhibit lipolysis in mice and human adipocytes. However, the function of CIDEC in regulation of milk lipid synthesis in dairy cow mammary gland remains largely unknown. In this study, 6 multiparous Holstein cows (parity = 3) in early lactation were allocated to high-fat milk (milk yield 33.9 +/- 2.1 kg/d, milk fat >3.5%, n = 3) and low-fat milk (milk yield 33.7 +/- 0.5 kg/d, milk fat <3.5%, n = 3) groups according to their milk fat content. Lactating cows were slaughtered at 90 d in milk and mammary tissues were collected to detect. CIDEC localization. Immunofluorescence staining of sections of lactating mammary glands with high-and low-fat milk showed that CIDEC was expressed in the cytoplasm of epithelial cells and localized to lipid droplets. Lipid droplets and CIDEC protein were also detected in isolated lactating mammary epithelial cells of dairy cows. Immunostaining of CIDEC in isolated mammary epithelial cells also confirmed its presence in the nucleus. The knockdown of CIDEC in cultured bovine mammary epithelial cells decreased milk lipid content and reduced expression of genes associated with mammary de novo fatty acid synthesis, short-and long-chain intracellular fatty acid activation, triacylglycerol synthesis, and transcription regulation. These genes included those for acetyl-CoA carboxylase (ACC, -60%), fatty acid synthase (FASN, -65%), acyl-CoA synthetase short-chain family member 2 (ACSS2, -50%), acyl-CoA synthetase long-chain family member 1 (ACSL1, -30%), diacylglycerol acyl-transferase 1 (DGAT1, -60%), sterol regulatory element-binding protein 1 (SBEBRI, -45%), and SBEBP cleavage activating protein (SOAP, -66%). Conversely, in cells overexpressing CIDEC, triacylglycerol content was increased, and transcription of those genes involved in milk lipid synthesis was coordinately upregulated. These results suggest that CIDEC plays an important role in regulating milk lipid synthesis in dairy cow Mainmary gland via a mechanism involving gene expression, which provides further insight into the mechanisms regulating mammary lipogenesis in ruminants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据