期刊
CHEMICAL SCIENCE
卷 11, 期 1, 页码 80-86出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9sc04823e
关键词
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资金
- National Natural Science Foundation of China [21635004, 21627806, 21705018]
- Fundamental Research Funds for the Central Universities [2242017K3DN11]
- State Key Laboratory of Analytical Chemistry for Life Science [SKLACLS1913]
- National Young 1000 Talents Program of China
- Jiangsu Province Education Department
- Southeast University-Nanjing Medical University Cooperative Research Project
- Jiangsu Province Innovative and Entrepreneurial Team
- Jiangsu Province Innovative and Entrepreneurial Talent
Accurate cancer cell identification and efficient therapy are extremely desirable and challenging in clinics. Here, we reported the first example of DNA tetrahedron nanostructures (DTNSs) to real-time monitor and image three intracellular miRNAs based on the fluorescence OFF to ON mode, as well as to realize cancer therapy induced by miRNA silencing. DTNSs were self-assembled by seven customized single-stranded nucleic acid chains containing three recognition sequences for target miRNAs. In the three vertexes of DTNSs, fluorophores and quenchers were brought into close proximity, inducing fluorescence quenching. In the presence of target miRNAs, fluorophores and quenchers would be separated, resulting in fluorescence recovery. Owing to the unique tetrahedron-like spatial structure, DTNSs displayed improved resistance to enzymatic digestion and high cellular uptake efficiency, and exhibited the ability to simultaneously monitor three intracellular miRNAs. DTNSs not only effectively distinguished tumor cells from normal cells, but also identified cancer cell subtypes, which avoided false-positive signals and significantly improved the accuracy of cancer diagnosis. Moreover, the DTNSs could also act as an anti-cancer drug; antagomir-21 (one recognition sequence) was detached from DTNSs to silence endogenous miRNA-21 inside cells, which would suppress cancer cell migration and invasion, and finally induce cancer cell apoptosis; the result was demonstrated by experiments in vitro and in vivo. It is anticipated that the development of smart nanoplatforms will open a door for cancer diagnosis and treatment in clinical systems.
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