期刊
CHEMICAL SCIENCE
卷 11, 期 1, 页码 281-289出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9sc03798e
关键词
-
资金
- National Natural Science Foundations of China [21778048, 21642007, 81473202, 81573411, 81225022]
- Zhejiang Provincial Natural Science Foundation of China [R18H300001, Z16H310003]
- National Science & Technology Major Project Key New Drug Creation and Manufacturing Program [2018ZX09711002010-004]
In vivo real-time imaging of nitrosative stress in the pathology of stroke has long been a formidable challenge due to both the presence of the blood-brain barrier (BBB) and the elusive nature of reactive nitrogen species, while this task is also informative to gain a molecular level understanding of neurovascular injury caused by nitrosative stress during the stroke episode. Herein, using a physicochemical property-guided probe design strategy in combination with the reaction-based probe design rationale, we have developed an ultrasensitive probe for imaging nitrosative stress evolved in the pathology of stroke. This probe demonstrates an almost zero background fluorescence signal but a maximum 1000-fold fluorescence enhancement in response to peroxynitrite, the nitrosative stress marker. Due to its good physicochemical properties, the probe readily penetrates the BBB after intravenous administration, and quickly accumulates in mice brain to sense local vascular injuries. After accomplishing its imaging mission, the probe is easily metabolized and therefore won't cause safety concerns. These desirable features make the probe competent for the straightforward visualization of nitrosative stress progression in stroke pathology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据