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Lessons from eRNAs: understanding transcriptional regulation through the lens of nascent RNAs

期刊

TRANSCRIPTION-AUSTIN
卷 11, 期 1, 页码 3-18

出版社

TAYLOR & FRANCIS INC
DOI: 10.1080/21541264.2019.1704128

关键词

RNA polymerase II; enhancers; nascent RNA; gene expression; non-coding RNA function; eRNAs; transcription factors; chromatin structure

资金

  1. NIH [GM125871]
  2. NSF [DBI 1759949]
  3. Sie Postdoctoral Fellowship

向作者/读者索取更多资源

Nascent transcription assays, such as global run-on sequencing (GRO-seq) and precision run-on sequencing (PRO-seq), have uncovered a myriad of unstable RNAs being actively produced from numerous sites genome-wide. These transcripts provide a more complete and immediate picture of the impact of regulatory events. Transcription factors recruit RNA polymerase II, effectively initiating the process of transcription; repressors inhibit polymerase recruitment. Efficiency of recruitment is dictated by sequence elements in and around the RNA polymerase loading zone. A combination of sequence elements and RNA binding proteins subsequently influence the ultimate stability of the resulting transcript. Some of these transcripts are capable of providing feedback on the process, influencing subsequent transcription. By monitoring RNA polymerase activity, nascent assays provide insights into every step of the regulated process of transcription.

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