期刊
JOURNAL OF CONTROLLED RELEASE
卷 263, 期 -, 页码 39-45出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2017.01.037
关键词
Targeted protein delivery; Lipidoids; Protein engineering; CD44; Hyaluronic acid
资金
- National Science Foundation [DMR 1452122]
- University Young Key Teachers Abroad Training Projects from the China Scholarship Council (CSC)
- NIH [OD021624]
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [1452122] Funding Source: National Science Foundation
Developing safe and effective nanosystems to deliver active and therapeutic proteins to targeted cells and organs is an important tool for many biomedical applications. We present here a simple and efficient strategy for this purpose: delivering hyaluronic acid (HA)-modified RNase A (RNase A-HA) in nanocomplex with cationic lipid-like molecules (lipidoids) to cancer cells, resulting in targeted inhibition of cancer proliferation. The chemical conjugation of RNase A with HA both increased the supramolecular interaction with carrier lipidoids, promoting protein encapsulation efficacy, and facilitated cancer cell targeting via interaction with overexpressed CD44. Through confocal laser scanning microscopy and flow cytometry analysis, we demonstrated that protein/lipidoid nanoparticles could facilely enter cells with high CD44 expression, and inhibit cell proliferation in a dose-dependent manner. (C) 2017 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据