期刊
JOURNAL OF CONTROLLED RELEASE
卷 248, 期 -, 页码 53-59出版社
ELSEVIER
DOI: 10.1016/j.jconrel.2017.01.008
关键词
Bone morphogenetic protein-2 (BMP-2); Fibroblast growth factor-2 (FGF-2); Diabetes mellitus; Polyethylenimine; Non-viral gene delivery; Bone regeneration
资金
- American Orthopaedic Foot Ankle Society
- Lyle and Sharon Bighley Professorship
Bone fracture healing impairment related to systemic diseases such as diabetes can be addressed by growth factor augmentation. We previously reported that growth factors such as fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 (BMP-2) work synergistically to encourage osteogenesis in vitro. In this report, we investigated if BMP-2 and FGF-2 together can synergistically promote bone repair in a leporine model of diabetes mellitus, a condition that is known to be detrimental to union. We utilized two kinds of plasmid DNA encoding either BMP-2 or FGF-2 formulated into polyethylenimine (PEI) complexes. The fabricated nanoplexes were assessed for their size, charge, in vitro cytotoxicity, and capacity to transfect human bone marrow stromal cells (BMSCs). Using diaphyseal long bone radial defects in a diabetic rabbit model it was demonstrated that codelivery of PEI-(pBMP-2+pFGF-2) embedded in collagen scaffolds resulted in a significant improvement in bone regeneration compared to PEI-pBMP-2 embedded in collagen scaffolds alone. This study demonstrated that scaffolds loaded with PEI-(pBMP-2+pFGF-2) could be an effective way of promoting bone regeneration in patients with diabetes. (C) 2017 Elsevier B.V. All rights reserved.
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