4.8 Article

Precise and combinatorial PEGylation generates a low-immunogenic and stable form of human growth hormone

期刊

JOURNAL OF CONTROLLED RELEASE
卷 249, 期 -, 页码 84-93

出版社

ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2017.01.029

关键词

Expanded genetic code; Copper-free click chemistry; Multisite specific PEGylation; Recombinant human growth hormone

资金

  1. National Natural Science Foundation of China [81530090, 81673342, 21572015]
  2. National Key Research and Development Program of China [2016YFA0501500]

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In this study, we aimed to develop a safe and stable form of human growth hormone (hGH) and to refine PEGylation methods for therapeutic proteins via genetic code expansion. Through this precise approach, a series of polyethylene glycol (PEG) moieties and sites were combined in various ways. Additionally, the effects of combinatorial PEGylation on the biological, pharmacological, and immunogenic properties of hGH in vitro and vivo were analyzed. Our results showed that combinatorial PEGylation at Y35, G131, and K145 significantly reduced immunogenicity and improved pharmacokinetic (PK) profiles compared with mono-PEGylation, while retaining biological activity. Upon re-examination of the pharmacodynamics in hypophysectomized rats, multi-PEGylated hGH was found to be much more stable than mono-PEGylated hGH. Thus, this method for combinatorial, precise PEGylation may facilitate the development of next-generation, long-acting hGH with low immunogenicity. (C) 2017 Elsevier B.V. All rights reserved.

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