期刊
BLOOD ADVANCES
卷 4, 期 1, 页码 28-39出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/bloodadvances.2019000845
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资金
- Agence Nationale de la Recherche (ANR) EpiSwitch 2016
- Societe Francaise d'Hematologie
- ANR Epi-Switch 2016
- University of Limoges (France)
- Region Nouvelle Aquitaine (France)
- Fondation Partenariale de l'Universite de Limoges
- Association Limousine pour l'Utilisation du Rein Artificiela Domicile
Numerous B-cell lymphomas feature translocations linking oncogenes to different locations in the immunoglobulin heavy chain (IgH) locus. During Burkitt lymphoma (BL), IgH breakpoints for c-myc translocation stand either close to J(H) segments or within switch regions. Transcription, accessibility, and remodeling of the IgH locus are under the control of the 2 potent cis-acting enhancer elements: E-mu and the 3' regulatory region (3'RR). To ensure their respective contributions to oncogene deregulation in the context of the endogenous IgH locus, we studied transgenic mice harboring a knock-in of c-myc in various positions of the IgH locus (39 to J(H) segments, 5' to C-mu with E-mu deletion and C-alpha). The observed spectrum of tumors, kinetics of emergence, and transcriptome analysis provide strong evidence that both E-mu and 3'RR deregulate c-myc and cooperate together to promote B-cell lymphomagenesis. Transgenics mimicking endemic BL (with c-myc placed 3' to J(H) segments) exhibited the highest rate of B-cell lymphoma emergence, the highest Ki67 index of proliferation, and the highest transcriptomic similarities to human BL. The 3'RR enhancer alone deregulated c-myc and initiated the development of BL-like lymphomas, suggesting that its targeting would be of therapeutic interest to reduce c-myc oncogenicity in vivo.
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