期刊
ACS APPLIED BIO MATERIALS
卷 3, 期 1, 页码 45-52出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.9b00842
关键词
fluorescent probe; near-infrared; ratiometric; aza-BODIPY; in vivo imaging
资金
- National Natural Science Foundation of China [21622602, 21788102, 21421004, 21636002, 21908060]
- National Key Research and Development Program [2017YFC0906902]
- Shanghai Municipal Science and Technology Major Project [2018SHZDZX03]
- Innovation Program of Shanghai Municipal Education Commission, Scientific Committee of Shanghai [15XD1501400]
- Shuguang Program [185G27]
- China Postdoctoral Science Foundation [2019M651417]
Precise in vivo tracking of hydrogen peroxide is still challenging due to its dynamic complexity and intrinsic background interference. Herein, we describe a rational design strategy to construct asymmetric aza-boron-dipyrromethane derivative (BODIPY)-based ratiometric probes for in vivo tracking H2O2, which are composed of a near-infrared aza-BODIPY core, active targeting group, and H2O2-specific recognition unit. We take advantage of two terminal functionalized conjunctions in the bis-condensed aza-BODIPY by rationally introducing carbonyl group as an electrondeficiency linker for regulating intramolecular charge transfer-induced wavelength shift and by attaching hydrophilic polyethylene glycol-biotin segment as the active targeting moiety. The probe BP5-NB-OB features several striking characteristics: (i) ratiometric near infrared response in both absorption and emission spectra; (ii) active targeting ability (biotin receptor-mediated endocytosis) with excellent biocompatibility; and (iii) in vivo tracking of endogenous H2O2. It was demonstrated that the probe BP5-NB-OB was successfully utilized for tracking endogenous H2O2 in living cells and tumorbearing mice, providing opportunities to insight into H2O2 related diseases for clinical application.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据