期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 508, 期 -, 页码 488-499出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2017.08.029
关键词
Cation-exchange membrane; beta-Lactoglobulin; Peptide sequence; Electrostatic and hydrophobic interactions; Peptide/peptide and peptide/membrane interactions
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- French National Research Agency (ANR) [ANR-11-EQPX-0037]
- Nord-Pas-de-Calais Region
- FEDER
- Agence Nationale de la Recherche (ANR) [ANR-11-EQPX-0037] Funding Source: Agence Nationale de la Recherche (ANR)
Polypeptide/solid charged surface interactions are omnipresent in the biomedical and biochemical fields. The present study aimed to understand the adsorption mechanisms of a cation-exchange membrane (CEM) by a well-characterized peptide mixture at three different pH values. Results demonstrated that fouling was important at pH 6, twice lower at pH 2 and negligible at pH 10. At pH 6, ALPMHIR and TKIPAVFK sequences firstly established electrostatic interactions with the negative CEM charges (SO3-) through their positive K and R residues (NH3+) creating a first nanolayer. Secondly, peptide peptide interactions occurred through their respective hydrophobic residues creating a second nanolayer. At pH 2, VLVLDTDYK and IDALNENK sequences interacted only electrostatically and that in a lower proportion since at acidic pH values, most of the CEM charges would be protonated and uncharged (HSO3) and then limit the potential electrostatic interactions. In addition, the sequences of peptides interacting at pH 2 and 6 were different. This was explained by their structure in terms of residue nature and position in the sequence. At pH 10, no fouling was observed due to the lack of positive peptide charges. To the best of our knowledge, it is the first indepth study concerning the fouling of CEMs by peptides from a complex mixture. (C) 2017 Elsevier Inc. All rights reserved.
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