期刊
JOURNAL OF CLINICAL PERIODONTOLOGY
卷 44, 期 5, 页码 530-539出版社
WILEY
DOI: 10.1111/jcpe.12710
关键词
chemokines; homing; migration; periodontal stem cells; tissue regeneration
资金
- National Research Foundation of Korea (NRF) - Korean government (MSIP) [2015R1A2A1A15053961]
- Ministry of Science, ICT & Future Planning [2012M3A9B2052521]
- National Research Foundation of Korea [2015R1A2A1A15053961] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
AimThe present study aimed to characterize the expression pattern of chemokines obtained from inflamed periodontal defects and to determine the characteristics of human periodontal-ligament stem cells (hPDLSCs) migrated by each specific chemokine. Materials and MethodsBoth inflamed and healthy periodontal tissues were obtained from periodontitis patients (n=11), and the chemokine expression levels were analyzed. The periodontal-tissue-specific chemokines were applied to healthy hPDLSCs from extracted teeth (n=3), with FGF-2 acting as a positive control. Cells were separated by selected chemokines using transwell method into migrated/unmigrated hPDLSCs. The characteristics of the hPDLSC subpopulation recruited by each chemokine were assessed, and gene expression pattern was analyzed by microarray. ResultsChemokines were categorized into three groups by specific patterns of appearing, increasing, and decreasing/disappearing from healthy to inflamed tissues. A representative chemokine from each group enhanced the capacities for colony formation and osteogenic/adipogenic differentiation while maintaining the surface markers of hPDLSCs. RANTES/CCL5 significantly increased the cellular migration of hPDLSCs, via enhancement of signaling pathways, regulation of the actin skeleton, and focal adhesion. ConclusionThe present study found a specific chemokine profile induced by inflammation in periodontal tissues, with RANTES/CCL5 appearing to play a role in the migration of hPDLSCs into inflammatory periodontal lesions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据