期刊
NANOSCALE
卷 12, 期 3, 页码 1948-1957出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9nr09152a
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资金
- National Natural Science Foundation of China [NSFC 31600809, 31800841, U1604177, U1804139]
- National Key Technologies R&D Program of China [2018YFA0209800]
- Program of China's 1000-Talents Plan
- Key Research Program in Colleges and Universities of Henan Province [19zx006]
- Australian Endeavour Fellowship [69172018]
- Mason Foundation National Medical Program [MAS2017F034]
- National Health and Medical Research Council (NHMRC) Dementia Fellowship [APP1111611]
Iron detection is one of the critical markers to diagnose multiple blood-related disorders that correspond to various biological dysfunctions. The currently available anemia detection approach can be used only for pre-treated blood samples that interfere with the actual iron level in blood. Real-time detection approaches with higher sensitivity and specificity are certainly needed to cope with the commercial level clinical analyses. Herein, we presented a novel strategy to determine the blood iron that can be easily practiced at commercial levels. The blend of well-known iron-cyanide chemistry with nanotechnology is advantageous with ultrahigh sensitivity in whole blood analysis without any pre-treatments. This approach is a combined detection system of the conventional assay (UV-visible spectroscopy) with surface-enhanced Raman scattering (SERS). Organic cyanide modified silver nanoparticles (cAgNPs) can selectively respond to Fe3+ ions and Hb protein with a detection limit of 10 fM and 0.46 mu g mL(-1), respectively, without being affected by matrix interfering species in the complex biological fluid. We confirmed the clinical potential of our new cAgNPs by assessing iron-status in multiple anemia patients and normal controls. Our SERS-based iron quantitation approach is highly affordable for bulk-samples, cheap, quick, flexible, and useful for real-time clinical assays. Such a method for metal-chelation has extendable features of therapeutics molecular tracking within more complex living systems at cellular levels.
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