期刊
JOURNAL OF CLINICAL NEUROSCIENCE
卷 36, 期 -, 页码 125-128出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jocn.2016.10.032
关键词
Polyethylene glycol; Peripheral nerve repair; PEG fusion; Flow cytometry; Neuroblastoma cells
资金
- Vanderbilt School of Medicine, Vanderbilt Ingram Cancer Center [P30 CA68485]
- Vanderbilt Digestive Disease Research Center [DK058404]
Polyethylene glycol (PEG) has long been used as a membrane fusogen, but recently it has been adopted as a technique for peripheral nerve repair. Vertebrate models using PEG fusion have shown improved outcomes when PEG is applied during repair of severed peripheral nerves. The cellular mechanism of PEG fusion in the peripheral nerve repair model has not previously been assessed via flow cytometry. PEG fusion was assessed in this experiment by dying B35 rat neuroblastoma cells with different color fluorescent labels. The different color cells were combined and PEG was applied in concentrations of 50%, 75% and 100%. The amount of cell fusion was assessed via flow cytometry as the percentage of double positive cells. Results showed increasing fusion and decreasing viability with increasing concentrations of PEG. (C) 2016 Elsevier Ltd. All rights reserved.
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