期刊
BIOLOGY-BASEL
卷 9, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/biology9010019
关键词
community composition; diatom; diversity; high throughput sequencing; molecular ecology; phytoplankton; metabarcoding
类别
资金
- National Science Foundation (NSF) [1638834, 1655686]
- University of Rhode Island
- NSF award [OIA-1655221]
- National Science Foundation EPSCoR awards [OIA-1655221, OIA-1004057]
- Directorate For Geosciences
- Division Of Ocean Sciences [1655686] Funding Source: National Science Foundation
- Division Of Ocean Sciences
- Directorate For Geosciences [1638834] Funding Source: National Science Foundation
Diatoms generate nearly half of marine primary production and are comprised of a diverse array of species that are often morphologically cryptic or difficult to identify using light microscopy. Here, species composition and realized thermal niches of species in the diatom genus Thalassiosira were examined at the site of the Narragansett Bay (NBay) Long-Term Plankton Time Series using a combination of light microscopy (LM), high-throughput sequencing (HTS) of the 18S rDNA V4 region and historical records. Thalassiosira species were identified over 6 years using a combination of LM and DNA sequences. Sixteen Thalassiosira taxa were identified using HTS: nine were newly identified in NBay. Several newly identified species have small cell diameters and are difficult to identify using LM. However, they appeared frequently and thus may play a significant ecological role in NBay, particularly since their realized niches suggest they are eurythermal and able to tolerate the >25 degrees C temperature range of NBay. Four distinct species assemblages that grouped by season were best explained by surface water temperature. When compared to historical records, we found that the cold-water species Thalassiosira nordenskioeldii has decreased in persistence over time, suggesting that increasing surface water temperature has influenced the ecology of phytoplankton in NBay.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据