期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 1068, 期 -, 页码 119-124出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2017.10.033
关键词
D4A; Abiraterone; LC-MS/MS; Therapeutic drug monitoring; Metabolite
Delta(4)-Abiraterone (D4A) is a recently discovered active metabolite of the oral anti-androgen drug abiraterone acetate. For quantification of this metabolite in human plasma, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated. Human plasma samples of patients treated with abiraterone acetate were prepared by protein precipitation with acetonitrile. The method was validated over a linear range of 0.2-20 ng/mL. Intra-assay and inter-assay variabilities were within +/- 15% of the nominal concentrations for quality control (QC) samples at medium and high concentrations and within +/- 20% at the lower limit of quantification (LLOQ), respectively. The described method for quantification of D4A was validated successfully and implemented to support therapeutic drug monitoring in patients treated with abiraterone acetate.
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