期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 1041, 期 -, 页码 37-44出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2016.11.029
关键词
N-Acylated-L-homoserine lactones (AHLs); Ultrasonic extraction (UE); Solid phase extraction (SPE); Quorum sensing (QS)
资金
- National High Technology Research and Development Program of China (863 Program) [2012AA063407]
- National Science & Technology Support Program of China [2014BAC08B04]
N-Acylated-L-homoserine lactones (AHLs) play a vital role in Gram-negative bacteria communication by promoting the formation of extracellular polymeric substances (EPS) and biofilms. However, the low concentration of these AHL signals makes the process difficult to understand. A robust and sensitive pretreatment method for AHL detection was developed in this work. Compared with eight different solid-phase extraction (SPE) columns and three various solid extraction method, we found that the UE (ultrasonic extraction) and an Oasis hydrophilic-lipophilic-balanced (HLB) sorbent in column format combined with ultra-performance liquid chromatography linked to tandem mass spectrometry (UPLC-MS/MS) can be successfully used for systematic pretreating moving bed biofilm reactor (MBBR) biological samples to extract AHLs and determine concentration of AHLs in wastewater treatment biofilms. This easy-to-follow protocol makes it ideal for quantitative analyses of AHLs in wastewater treatment biofilms. (C) 2016 Elsevier B.V. All rights reserved.
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