期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1530, 期 -, 页码 204-210出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2017.11.042
关键词
Sample preparation; Solvent Front Position Extraction; Matrix effect
资金
- Operational Program Development of Eastern Poland, Priority Axis I, Modern Economy, Operations 1.3. Innovations Promotion [POPW.01.03.00-06-010/09-00]
A concept of using thin-layer chromatography to multicomponent sample preparation for quantitative determination of solutes followed by instrumental technique is presented. Thin-layer chromatography (TLC) is used to separate chosen substances and their internal standard from other components (matrix) and to form a single spot/zone containing them at the solvent front position. The location of the analytes and internal standard in the solvent front zone allows their easy extraction followed by quantitation by HPLC. (C) 2017 Elsevier B.V. All rights reserved.
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