期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1499, 期 -, 页码 132-139出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2017.03.086
关键词
Danshen injection; Ionic liquids; Liquid-in-water microemulsion; Microemulsion liquid chromatography; Phenolic acids; Salvia miltiorrhiza
资金
- Zhejiang Provincial Natural Science Foundation of China [LY15H280016]
- General Program of National Natural Science Foundation of China [81573552]
- Hangzhou social development of scientific research projects [20150533B05]
An environmentally friendly ionic liquid-in-water (IL/W) microemulsion was established and applied as mobile phase in microemulsion liquid chromatography (MELC) with ultraviolet (UV) detection or electrochemical detector (ECD) for analysis of phenolic compounds in real samples. The optimal condition of the method was using the best composition of microemulsion (0.2% w/v [HMIM]PF6, 1.0% w/v SDS, 3.0% w/v n-butanol, 95.8% v/v water, pH 2.5) with UV detection. The validation results indicated that the method provided high degree of sensitivity, precision and accuracy with the low limit of detections ranged from 17.9-238 ng/mL, satisfactory mean recovery values in the range of 80.1-105% and good linearity (r(2) > 0.9994). Additionally, this method exhibited high selectivity and resolution for the analytes and was more eco-friendly compared with traditional MELC method. Consequently, the established IL/W MELC method was successfully applied to simultaneously separate and determine target compounds in Danshen sample and its preparation. (C) 2017 Elsevier B.V. All rights reserved.
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