期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1529, 期 -, 页码 1-28出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2017.11.003
关键词
Capillary electrophoresis; enzymatic assays; high performance liquid chromatography; microscale thermophoresis; kinetic constants
资金
- Region Centre (France)
Elastase, collagenase, hyaluronidase and tyrosinase, are very interesting enzymes due to their direct implication in skin aging and as therapeutic hits. Different techniques can be used to study these enzymes and to evaluate the influence of effectors on their kinetics. Nowadays, analytical techniques have become frequently used tools for miniaturizing enzyme assays. The main intention of this article is to review chromatographic and electrophoretic tools that study the four enzymes above mentioned. More specifically, the use of high-performance liquid chromatography and capillary electrophoresis and their derivative techniques for monitoring these enzymes will be investigated. The advantages and limitations of these assays will also be discussed. The original use of microscale thermophoresis and thin layer chromatography in this domain will also be covered. (C) 2017 Elsevier B.V. All rights reserved.
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