期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1529, 期 -, 页码 57-62出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2017.10.076
关键词
Anatoxin-a; Cyanobacteria; Antibody; Magnetic; Immunoaffinity; Ion mobility spectrometry
资金
- Ministerio de Economia y Competitividad [CTQ 2014-52841-P, AGL2015-64488-C2-1 /2-R]
- Generalitat Valenciana [PROMETEO-II 2014-077]
Specific monoclonal antibodies were coupled with magnetic Sepharose-based beads and used, for the first time. The methodology was applied to preconcentrate anatoxin-a from water and the later determination by ion mobility spectrometry (IMS). Dispersive magnetic immunoaffinity (d-MagIA) extraction methodology provided a limit of detection of 0.0214 L-1 and a satisfactory precision with a relative standard deviation lower than 15%. Recoveries were evaluated at 0.5, 1.0 and 5.0 mu g L-1 anatoxin-a with quantitative values from 91 to 115%. Additionally, isobaric interferences with phenylalanine were completely avoided by the use of the developed d-MagIA extraction coupled to IMS determinations. (C) 2017 Elsevier B.V. All rights reserved.
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