期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1523, 期 -, 页码 40-48出版社
ELSEVIER
DOI: 10.1016/j.chroma.2017.07.055
关键词
Microscale separations; Nanoproteomics; NanoLC; Capillary electrophoresis; Mass spectrometry
资金
- NIH [P41 GM103493, DP3 DK110844, UC4 DK104167]
- DOE
- DOE [DE-AC05-76RL0 1830]
Microscale separation (e.g., liquid chromatography or capillary electrophoresis) coupled with mass spectrometry (MS) has become the primary tool for advanced proteomics, an indispensable technology for gaining understanding of complex biological processes. In recent decades significant advances have been achieved in MS-based proteomics. However, the current proteomics platforms still face an analytical challenge in overall sensitivity towards nanoproteomics applications for starting materials of less than 1 mu g total proteins (e.g., cellular heterogeneity in tissue pathologies). Herein, we review recent advances in microscale separation techniques and integrated sample processing strategies that improve the overall sensitivity and proteome coverage of the proteomics workflow, and their contributions towards nanoproteomics applications. (C) 2017 Elsevier B.V. All rights reserved.
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