期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1524, 期 -, 页码 153-159出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2017.10.005
关键词
Hydrophilic interaction liquid chromatography; Polyacrylamide; Carbohydrates; Alcohols
资金
- Project of National Natural Science Foundation of China [81403100]
- Fundamental Research Funds for the Central Universities [222201717016]
- Open Funding Project of State Key Laboratory of Microbial Metabolism
A hydrophilic interaction liquid chromatography (HILIC) stationary phase was prepared by a two-step synthesis method, immobilizing polyacrylamide on silica sphere particles. The stationary phase (named PA, 5 mu m dia) was evaluated using a mixture of carbohydrates in HILIC mode and the column efficiency reached 121,000 N m(-1). The retention behavior of carbohydrates on PA stationary phase was investigated with three different organic solvents (acetonitrile, ethanol and methanol) employed as the weak eluent. The strongest hydrophilicity of PA stationary phase was observed in both acetonitrile and methanol as the weak eluent, when compared with another two amide stationary phases. Attributing to its high hydrophilicity, three oligosaccharides (xylooligosaccharide, fructooligosaccharide and chitooligosaccharides) presented good retention on PA stationary phase using alcohols/water as mobile phase. Finally, PA stationary phase was successfully applied for the purification of galactooligosaccharides and saponins of Paris polyphylla. It is feasible to use safer and cheaper alcohols to replace acetonitrile as the weak eluent for green analysis and purification of polar compounds on PA stationary phase. (C) 2017 Published by Elsevier B.V.
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