期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1498, 期 -, 页码 8-21出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2017.01.002
关键词
Review; Stationary phases; Capillary columns; Enzymatic reactors; Two-dimensional liquid chromatography; Biomolecule analysis; Spatial chromatography
资金
- Flemish Agency of Innovation and Entrepreneurship [IWT.150467]
- Chinese Fundamental Research Funds for the Central Universities [buctrc201413]
This review focuses on the preparation of organic polymer-based monolithic stationary phases and their application in the separation of biomolecules, including antibodies, intact proteins and protein isoforms, oligonucleotides, and protein digests. Column and material properties, and the optimization of the macropore structure towards kinetic performance are also discussed. State-of-the-art liquid chromatography-mass spectrometry biomolecule separations are reviewed and practical aspects such as ion-pairing agent selection and carryover are presented. Finally, advances in comprehensive two-dimensional LC separations using monolithic columns, in particular ion-exchange x reversed-phase and reversed-phase x reversed-phase LC separations conducted at high and low pH, are shown. (C) 2017 Elsevier B.V. All rights reserved.
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