期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1488, 期 -, 页码 93-103出版社
ELSEVIER
DOI: 10.1016/j.chroma.2017.01.088
关键词
Haematococcus pluvialis; Carotenoid profiling; Stationary phase; C18 column; Environmental stress
资金
- S&T Projects at the Shenzhen Science and Technology Innovation Committee [JCYJ20150831192329178, JSGG20140519113458237, CXZZ20150529165045063, KQJSCX2016022619041970]
A simple ultra-high-pressure liquid chromatography (UHPLC) method for rapidly and simultaneously identifying thirteen carotenoids in Haematococcus pluvialis was developed in this study. The method is capable of effectively separating two astaxanthin isomers, two zeta-carotene isomers, and three phytoene isomers on two simple C18 columns within 9 and 12 min only by using methanol and acetonitrile, respectively. To our best knowledge, this is the rapidest method for these carotenoid isomers, currently. Using this method, carotenoid profiling in the astaxanthin-accumulating H. pluvialis under environmental stresses was successfully carried out. Results indicated that carotenoid biosynthesis was differentially perturbed by environmental stresses, indicating that this simple and rapid method is suitable to not only bacterial but also algal samples, with potential applications for a wide range of samples from plant to animal. Finally, possible reasons for the elution order of carotenoids were studied. (C) 2017 Elsevier B.V. All rights reserved.
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