期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1507, 期 -, 页码 141-148出版社
ELSEVIER
DOI: 10.1016/j.chroma.2017.05.047
关键词
Blood smears; Microfluidics; Deterministic migration; Microfabricated grid film; Cytochemical analysis
资金
- Pioneer Research Center Program through the National Research Foundation (NRF) of Korea - Ministry of Science, ICT & Future Planning [2013M3C1A3064777]
- NRF grant - Korea government (MSIP) [2014R1A2A2A09052449, 2015R1C1A1A01053990]
- National Research Foundation of Korea [2015R1C1A1A01053990] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Cytological examination of peripheral white blood cells inhomogeneously distributed on a blood smear is currently limited by the low abundance and random sampling of the target cells. To address the challenges, we present a new approach to prepare and analyze concentrated blood smears by rapidly enriching white blood cells up to 32-fold with 92% recovery on average at a high throughput (1 mL/min) using a deterministic migration-based separator and by systematically analyzing a large number of the cells distributed over a blood slide using a microfabricated grid film. We anticipate that our approach will improve the clinical utility of blood smear tests, while offering the capability to detect rare cell populations. (C) 2017 Elsevier B.V. All rights reserved.
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