期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1519, 期 -, 页码 145-151出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2017.08.053
关键词
Two dimensional liquid chromatography; Sephadex LH-20 x reversed-phase liquid; chromatography; Mixed-mode; Flos Carthami; Carthamus tinctorius; Flavonoids
资金
- Key Projects of Fujian Science and Technology Program [2014Y0043]
- Special programs of Marine Public Welfare Profession [201405038]
- Scientific Research Foundation of Third Institute of Oceanography, SOA [2013005]
- Engineering Research Center of Marine Bioresources Comprehensive Utilization, SOA [MBRCU201603]
A novel on-line comprehensive two-dimensional liquid chromatography (2D-LC) method by coupling mixed-mode gel liquid chromatography (MMG-LC) with reversed phase liquid chromatography (RPLC) was developed. A mixture of 17 reference compounds was used to study the separation mechanism. A crude water extract of Flos Carthami was applied to evaluate the performance of the novel 2D-LC system. In the first dimension, the extract was eluted with a gradient of water/methanol over a cross linked dextran gel Sephadex LH-20 column. Meanwhile, the advantages of size exclusion, reversed phase partition and adsorption separation mechanism were exploited before further on-line reversed phase purification on the second dimension. This novel on-line mixed-mode Sephadex LH-20 x RPLC method provided higher peak resolution, sample processing ability (2.5 mg) and better orthogonality (72.9%) versus RPLC x RPLC and hydrophilic interaction liquid chromatography (HILIC) x RPLC. To the best of our knowledge, this is the first report of a mixed-mode Sephadex LH-20 x RPLC separation method with successful applications in on-line mode, which might be beneficial for harvesting targets from complicated medicinal plants. (C) 2017 Elsevier B.V. All rights reserved.
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